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KMID : 0613820050150040566
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Journal of Life Science
2005 Volume.15 No. 4 p.566 ~ p.571
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Characteristics of Heavy Metal Resistant Plasmid in Enterobacter cloaceae K41
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Kim Young-Hee
Lee Sang-Joon
Chung Kyung-Tae
Jeong Young-Kee
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Abstract
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A natural habitat bacterium, Enterobacter cloaceae K41 was isolated from fresh water plant root and identified. This strain was used to investigate heavy metal resistance. The optimal growth conditions of the bacterium were LB medium containing 1% yeast extract, 1% lactose, 1% NaCl, pH 7.0, at 37℃, and for 24 hours on a shaker. The minimal inhibitory concentration (MIC) of heavy metals against E. cloaceae KCTC2519 and E. cloaceae K41 was compared. The MIC of E. cloaceae K41 was 150 ppm in Cu, 50 ppm in Cd whereas that of the standard strain was 50 ppm in Cu but no growth was observed either Cd or two mixed heavy metal solution. The presence of plasmid was cleared from the isolated strain whereas no possession from the standard strain. The plasmid from E. cloaceae K41 was transformed into E. coli DH5α. The MIC of transformed strain increased resistance 7 times in Cu and 6 times in Cd by insertion of this plasmid. The metal adsorption of the transformant was increased 1.3 times in Cu and 1.5 times in Cd indicating the plasmid was responsible for heavy metal resistance.
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